To evaluate Phytochemical and antimicrobial activity of different parts (Flower & Leaf) of Tagetes erecta, Rosa, Nerium oleander. Different Part of Tagetes erecta, Rosa, Nerium oleander were extracted with 20% , 30%, 50% (v/v) solvent (Acetone, Methanol, Ethanol). The Vibrio sps (V. parahaemolyticus & V. vulnificus). used in this study were obtained on TCBS (Thiosulfate Citrate Bile sucrose) Media. The plant extracts were subjected to antimicrobial assay. In case of antimicrobial screening, crude extract of flower & leaves showed notable antibacterial activity against tested microorganisms. In Antibacterial Activity of the plant extract showed maximum zone of Inhibition was observed in 50 % Rose Leaves Acetone extract (28 mm) and minimum zone of inhibition was observed in 20 % Rose Flower Methanol extract (10 mm). From this study, it can be concluded that the species is effective in scavenging free radicals and has the potential to be a powerful antioxidant. These plant extracts which proved to be potentially effective can be used as natural alternative preventives to control cholera diseases and preserve food stuff avoiding healthy hazards of chemically antimicrobial agent applications.