Background: Propolis commonly known as bee glue, is a wax-cum-resin substance collected by honeybees from various plant sources. Active ingredients from the propolis are flavonoids, phenolics, terpenes, cinnamic acid, caffeic acid, fatty acids, vitamin A, B complex vitamins and essential oils. Due to these compositions it has wide range of biological activities including antibacterial, antiviral, fungicidal, anti-inflammatory as well as antioxidative. Aim: To study effect of bee propolis in controlling plaque & gingivitis by using 3.5% ethanolic extract of bee propolis in Gel form. Materials and Method: Purposive samples were selected which comprised of 30 adult patients, both women and men. Group I included 15 patients who were kept on local application of propolis gel without following oral prophylaxis. Group II included 15 patients who were kept on local application of propolis gel after scaling and polishing. Base-line indices were scored & recorded in both groups. In both groups score were recorded at end of second, third & fourth week. All patients were asked to apply 3.5% propolis gel to gingiva & teeth surface twice daily in morning after brushing & at night prior sleeping. Results: Plaque and Gingival index was assessed among the Groups at baseline, at 2nd week, 3rd week & at 4th week. The highly significant difference was found amongst the groups (p<0.00). Conclusion: The present study showed evidence of the efficacy of 3.5% ethanolic extract of Indian propolis is used in controlling plaque & gingivitis.