Rp-hplc method development and validation for the simultaneous estimation of sofosbuvir and velpatasvir in bulk and pharmaceutical dosage form

A simple, Accurate, precise method was developed for the simultaneous estimation of the Sofosbuvir and Velpatasvir in Tablet dosage form. Chromatogram was run through Std BDS 250 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 240.0 nm. Retention time of Sofosbuvir and Velpatasvir were found to 2.251 min and 2.633. %RSD of the Sofosbuvir and Velpatasvir were and found to be 0.4 and 0.4 respectively. %Recovery was obtained as 99.11% and 98.81% for Sofosbuvir and Velpatasvir respectively. LOD, LOQ values obtained from regression equations of Sofosbuvir and Velpatasvir were 0.16, 0.48 and 1.15, 3.47 respectively. Regression equation of Sofosbuvir is y = 1907.x+10654, y = 4963x+9760 of Velpatasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.