The growing demand for natural coagulants led to the necessity for alternate rennet substitutes, searching a new sources of proteolytic enzymes with coagulant properties. The utilization of waste as a raw material is very important for the natural balance. In this study, the distillery waste the distillers yeast sludge was used as a fermentation medium in the Production of Milk-Clotting Enzyme in submerged fermentation by Streptococcus lactis. The synthetic fermentation medium was optimized by using various carbon and nitrogen sources in both the stationary and shaking conditions then the production was carried out in distillers medium with optimized conditions under shaking at 120rpm. The maximum milk clotting activity of 0.625 units/mg and the proteolytic activity of 0.490 units/mg were obtained at 40° C. The kinetic parameters Michaelis–Menten and Lineweaver‐Burk plots were evaluated in this study.
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2022: 6.012
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