The present experiment was conducted to measure the effectiveness of different media on the growth and antagonistic effect of biocontrol agents against some fungi. Five numbers of fungal isolates Fusarium equseti, Fusarium acuminatum, Colletotrichum gloeosporioides, Fusarium javanicum, Nigrospora sphaeria were tested against five numbers of biocontrol fungi Penicillium citrinum, Penicillium oxalicum, Aspergillus ochraceus, Penicillium capsulatum, Talaromyce cnidii by dual culture technique in five different nutrition media such as Saboroud Dextrose (SD), Czapek’s Dox (CZ), Potato Dextrose (PD), Malt Extract (ME), Oatmeal agar (OA). Observation was taken by measuring the zone of inhibition along with the radial growth of both of the fungal isolates. Results obtained that the highest inhibition zones, i.e., 1.6 cm, 1.2 cm, 1.2 cm, 3cm and 0.7 cm, observed in PD, SD, SD, and ME media, respectively, in the case of five biocontrol agents (P. citrinum, P. oxalicum, A. ochraceus, P.capsulatum, T. cnidii) were significantly exhibited the strongest antagonism against the fungus F. equiseti. In case of Fusarium acuminatum, highest inhibition zones, i.e., 1.8 cm, 1.5 cm, 1.5 cm, 1.7cm and 4.0 cm, observed in ME, SD, SD, PD and PD media, respectively, against five biocontrol agents (P. citrinum, P. oxalicum, A. ochraceus, P.capsulatum, T. cnidii). These agaents were significantly exhibited the strongest antagonism against the fungus F. acuminatum. In case of C. gloeosporioides, highest inhibition zones, i.e., 1 cm, 0.8 cm, 1 cm, 1.2cm and 1cm, observed in ME, SD, PD, ME, and PD media, respectively, against five biocontrol agents (P. citrinum, P. oxalicum, A. ochraceus, P.capsulatum, T. cnidii). These agaents were significantly exhibited the strongest antagonism against the fungus C. gloeosporioides. In case of F. javanicum, highest inhibition zones, i.e., 1.6 cm observed in ME media and other four 1.5 cm, 1 cm, 2.8cm and 0.5cm observed in SD media, respectively, against five biocontrol agents (P. citrinum, P. oxalicum, A. ochraceus, P.capsulatum, T. cnidii). These agaents were significantly exhibited the strongest antagonism against the fungus F. javanicum. . In case of N. sphaeria, highest inhibition zones, i.e., 2.3 cm, 1 cm, 1 cm, 2cm and 1.1cm, observed in PD, PD, ME, CZ, and PD media, respectively, against five biocontrol agents (P. citrinum, P. oxalicum, A. ochraceus, P.capsulatum, T. cnidii). These agaents were significantly exhibited the strongest antagonism against the fungus N. sphaeria. CZ and OMA media was having less impact on growth of fungi and also not giving any effect on the antagonistic behavior of biocontrol fungi as compared to other nutrient media.
Impact Factor
2022: 6.012
This work is licensed under a