Fresh and refrigerated conservation of boar semen with antioxidants

Oxidative stress affects the seminal conservation, mainly in pig sperm, since in these they are more susceptible to the effect of reactive oxygen species. The objective of this work was to assess the effect of the addition of vitamin C and E as antioxidants on motility, viability and acrosomal integrity (NAR)) of boar semen preserved in fresh and refrigerated. A sample of boar ejaculate of the York commercial breed was used, which was diluted with a commercial diluent and vitamins C, E and C + E in concentrations of 1, 2 and 4 mg / ml, to later keep it fresh and in Refrigeration for 24 hours. Motility, viability and acrosomal integrity (NAR) were assessed. The best results were: with vitamin C in a concentration of 1 mg / ml, 46%, 76% and 95% of motility, viability and of NAR when it was kept fresh; when it was kept under refrigeration, 10%, 57% 55% motility, viability and NAR were obtained, respectively. With vitamin E at a concentration of 2mg / ml stored fresh, 66%, 93% and 80% of motility, viability and NAR were obtained, respectively; preserved in refrigeration, 73%, 63% and 68% of NAR were obtained, respectively. When the combination of vitamins C + E was used at a concentration of 2 mg / ml stored fresh, 13%, 85% and 82% of motility, viability and NAR were obtained. When the same concentration of vitamins E + C was used in refrigeration, 0%, 33% and 26% of motility, viability and NAR were obtained, respectively. In conclusion, the addition to the diluent of vitamins C, E and C + E as antioxidants, offer promising results for fresh and chilled conservation of boar semen.