Aim:The Aim of this study is to evaluate and compare the effect of 17% liquid EDTA, 16% Sodium Gluconate and 9% HEBP on the microhardness of root dentin. Materials and Methods:Thirty mandibular premolars were selected and randomly assigned to one of the three groups(n = 10). Decoronate the teeth up to CEJ, specimens were prepared up to F3 (ProTaper Universal). Specimens were sectioned longitudinally to expose root dentin surface. The test group was one half, and the control group was the other half. Group 1: 17% liquid EDTA, Group 2: 16% Sodium gluconate, and Group 3: 9% HEBP. Measurement of microhardness was done using Vickers indenter with a load of 200 g and a holding time of 10 seconds. Statistical analysis: The data obtained was Statistical analyzed by using one-way ANOVA and post-hoc Tukey test at P ≤ 0.05. Results: The mean difference in microhardness of EDTA is more followed by sodium gluconate and HEBP. Conclusion:Using all three chelating agents resulted in a noticeable decrease in microhardness. HEBP, on the other hand, reduced microhardness the least and may be a useful chelating agent in endodontics.
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