Antibacterial activity against bacteria isolated from leaves of tabernaemontana divaricata

The ability of most infectious microbes evolving antimicrobial resistance necessitates discovery of novel antimicrobial agents from natural sources. For this study, a phytopathogen was isolated from infected leaf of T. divericata and identified as Pseudomonas aeruginosa with the standard methods. Antibacterial activity of standard drug i.e. ciprofloxacin and isolated compound from aqueous leaf extract of T. divericata was done against three phytopathogens i.e. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus in different dilutions (30µg/ml, 20 µg/ml, 10µg/ml) were studied. This study revealed that the isolated compound from aqueous extract of leaves of T. divaricata has capacity to act against phytopathogens. Isolated compound from aqueous extract of leaf showed resistant capacity against plant bacteria i.e. P. aeruginosa, E. coli and S.aureus through inhibition zone. Observation of results confirms that the 30 µg/ml concentration of isolated compound from aqueous extract of leaves of T. divaricata increased there is the possibility to observe more maximum inhibition against E. coli than P. aeruginosa and S.aureus bacterial species.