Wnt10a (rs 1054630) gene polymorphism and its association with human tooth agenesis in south indian population - using dna sequencing

Context: Non-syndromic tooth agenesis is a congenital defect without any other structural or developmental abnormalities.
Aims and Objectives: To evaluate the relationship, degree of association of WNT10A gene with Non syndromic tooth agenesis in in South Indian population.
Methods: DNA samples of 25 subjects with non-syndromic tooth agenesis and 25 unrelated controls, collected from the department were used for the study.
Group A: - DNA samples of 25 subjects with Non -syndromic tooth agenesis (T1 - T25)
Group B :- DNA samples of 25 unrelated controls (C1 - C25).
The extracted DNA samples were subjected to Polymerase chain reaction in which amplification of the selected gene segments was carried out; later these amplified products were subjected to DNA sequencing. Results were documented in the form of electropherograms.
Results: The results indicated that there is no association between the presence of WNT10A gene variant rs id 1054630 with the incidence of non syndromic tooth agenesis. This study also suggests that the likelihood of Non syndromic tooth agenesis is lower in subjects having AA (p=1.00) genotype.
Conclusion: This study indicates that there is an insignificant association between the presence of WNT10 A (rs 1054630) with the incidence of Non syndromic Tooth Agenesis in South Indian population.